REAL TIME PCR
(Real-time PCR) is commonly used to measure Gene Expression It is more sensitive than microarrays in detecting small changes in expression but requires more input RNA and is less adaptable to high-throughput studies, It is best suited for studies of small subsets of genes. Its one major shortcoming is that the sequence of the specific target gene of interest must be known (so you can design the PCR primers), hence real-time PCR can only be used for studying known genes.
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